A quantitative and qualitative evaluation of haematopoiesis in three habitat specialist fishes, Acanthocobitis botia, Devario aequipinnatus and Barilius barna from a hill stream environment
The aim of the present study was to execute quantitative and qualitative assessments of developing blood cells in the head kidney of three habitat specialist fish species of River Murti Acanthocobitis botia, Devario aequipinnatus and Barilius barna. The variance in activity pattern and preference of specific habitat of these three fishes were correlated with their haematopoietic profile. Eleven types of hematopoietic precursors and mature cells in erythroid and lymphoid lineage were recognized in head kidneys of the three fish species. The erythroid lineage was found to be the most abundant as well as diverse one with a special focus on the size and quantity of the small lymphoid haematoblasts, young and mature erythrocytes. Significant difference regarding the rate of maturation of developing blood cells and erythropoietic efficiency was found between these three species through cytological descriptions. These findings were corroborated through cell cycle study by flow cytometry analysis, which showed high, medium and low values of S-phase cells in A. botia, B. barna and D. aequipinnatus respectively, which corresponded to the turnover number that are associated with their respective habitat. The differential pattern of erythropoiesis might indicate its functional significance in the employment of different survival strategies that dictate varied activity and specialisation of habitat in fishes.
Acanthocobitis botia, Barilius barna, Devario aequipinnatus, Haematopoiesis, Head kidney, Survival strategy.