Human and Animal Studies   If the work involves chemicals, procedures or equipment that have any unusual hazards inherent in their use, the author must clearly identify these in the manuscript. If the work involves the use of animal or human subjects, the author should ensure that the manuscript contains a statement that all procedures were performed in compliance with relevant laws and institutional guidelines and that the appropriate institutional committee(s) has approved them. Authors should include a statement in the manuscript that informed consent was obtained for experimentation with human subjects. The privacy rights of human subjects must always be observed. And Also International Journal of Zoology and Applied Biosciences followed three guidelines of CPCSEA (Committee for the Purpose of Control and Supervision of Experiments on Animals), National Committee for Research Ethics in Science and Technology (NENT). Institutional Animal Ethics Committee (IAEC).