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International Journal of Zoology and Applied Biosciences Research Article

Study of pollen reproductive quality for In vitro haploid production in maize (Zea Mays L.) In Côte D'Ivoire

SORO Chigata Lohona, YAPO Sopie Edwige-Salomé, KOUADIO Oi Kouadio Samuel, KOUADIO Yatty Justin

Year : 2025 | Volume: 10 | Issue: 2 | Pages: 11-18

doi: https://doi.org/10.55126/ijzab.2025.v10.i02.002

Received on: 27/01/2025

Revised on: 12/02/2025

Accepted on: 25/02/2025

Published on: 31/03/2025

  • SORO Chigata Lohona, YAPO Sopie Edwige-Salomé, KOUADIO Oi Kouadio Samuel, KOUADIO Yatty Justin( 2025).

    Study of pollen reproductive quality for In vitro haploid production in maize (Zea Mays L.) In Côte D'Ivoire

    . International Journal of Zoology and Applied Biosciences, 10( 2), 11-18.

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Abstract

Maize is one of the most widely grown cereals in Côte d'Ivoire for both human and animal consumption. However, despite its nutritional and economic importance, its production faces challenges, such as climate change and soil degradation. To create new drought-resistant varieties, double-haploid technology was used in our breeding program using EV8728 maize. This study aimed to assess the reproductive quality of pollen and its ability to germinate in vitro. To this end, pollen grains collected from the experimental plot at Lorougnon GUEDE University were stored at 3 ºC and -26 ºC, then cultivated on modified Yu-Pei in vitro germination medium. The acetocarmin (1%) test on pollen harvested between 9 h and 11 h and stored for 1 month at 3 °C gave a high viability rate of 98.82% and 98.77%, respectively. In vitro germination was optimal on modified Yu-Pei medium at pH 6 supplemented with 25 g/l sucrose. Moreover, incubation in the dark at 30 ºC for 24 hours resulted in the highest pollen germination rate (97%). Pollen grains stored at 3 °C achieved the highest germination rates. Based on these results, the availability of pollen for the implementation of haploid in vitro methods in our breeding program is stable.

Keywords

Cereal, Haplo in vitro methods, In vitro germination, Plant breeding, Preservation, Viability.

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