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International Journal of Zoology and Applied Biosciences Research Article
Phytochemical screening, FT-IR, GC-MS characterization and antimicrobial activity of Pandanus amaryllifolius Roxb. Leaf extract
R. Sathish Kumar, L. Umaralikhan, R. Ranjani, S. Vasantha and R. Ravikumar
Year : 2026 | Pages: 12-24
Received on: 18/03/2026
Revised on: 22/04/2026
Accepted on: 14/05/2026
Published on: 30/05/2026
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R. Sathish Kumar, L. Umaralikhan, R. Ranjani, S. Vasantha and R. Ravikumar( 2026).
Phytochemical screening, FT-IR, GC-MS characterization and antimicrobial activity of Pandanus amaryllifolius Roxb. Leaf extract
. International Journal of Zoology and Applied Biosciences, 11( 3), 12-24.
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Abstract
The present study aimed to evaluate the presence of phytochemicals and antibacterial activity of the ethyl acetate extract of Pandanus amarylifolius leaves. Preliminary phytochemical screening revealed the presence of importance secondary metabolites such as phenols, alkaloids, flavonoids and some other bioactive compounds, which indicates the therapeutic potential of the plants. Fourier Transform Infrared (FT-IR) spectral analysis of ethyl extract of leaf depicted 12 functional groups peaks viz., Carboxylic acid (O-H), hydroxyl (O-H), alkane (C-H), Isothiocyanate (N=C=O) and Nitrile (C?O). Gas chromatography and mass spectroscopy analysis leaf extracts exhibited 26 compounds, including ?-Sitosterol, 1,2,3,6-Tetrahydrobenzylalcohol, ?- Amyrone, 24-Noroleana-3,12-diene, Neophytadiene, Benzene diazonium, Phenol, Cyclohexanol, 4-Norursa-3,12-diene, Brasiliamide, 9-Octadecenoic acid, Epilupeol, 4-Benzyl-2-t-butyl-3-formyloxazolidine-4-carboxylic acid, Benzene, Z-7-Decen-1-yl acetate, 6,9,12-Octadecatrienoic, Butanoic acid, Cyclopentane, 2(5H)-Furanone, Propanamide, 2-Butenoic acid, Cyclopentasiloxane, 3-Methylsalicylic acid, Cyclohexasiloxane and 5-Tetramethylhexane. These phytochemicals revealed significant antibacterial activity. The highest inhibition zone was observed against Staphylococcus aureus (29.25±0.35 mm) and Bacillus subtilis (28.95±0.35 mm) at a concentration of 500 µg/ml. The gram-negative bacteria Aeromonas hydrophila (25.45±0.35) and Klebsiella pneumonia (24.60±0.14) exhibited moderate inhibition zones. In the present study, the ethyl acetate extract of P. amaryllifolius leaves confirms significant phytochemical and promising antimicrobial potential, which can be used as a natural therapeutic source.
Keywords
Pandanus amaryllifolius, β -Sitosterol, Isothiocyana?te, Bacillus subtilis, Klebsiella pneumonia.
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© The Author(s) 2025. This article is published by International Journal of Zoology and Applied Biosciences under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
