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International Journal of Zoology and Applied Biosciences Research Article

Formulation and evaluation of polyherbal shampoo

T. Kavya, G. Nandhu, G. Yamini, K. Pranay, K. Jahnavi, K. Mamatha

Year : 2026 | Volume: 11 | Issue: 3 | Pages: 175-179

doi: https://doi.org/10.55126/ijzab.2026.v11.i03.027

Received on: 15/02/2026

Revised on: 26/02/2026

Accepted on: 23/04/2026

Published on: 01/05/2026

  • T. Kavya, G. Nandhu, G. Yamini, K. Pranay, K. Jahnavi, K. Mamatha( 2026).

    Formulation and evaluation of polyherbal shampoo

    . International Journal of Zoology and Applied Biosciences, 11( 3), 175-179.

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Abstract

Since ancient times, humans have relied on natural ingredients for health and hair care due to their protective and corrective properties. While synthetic shampoos are effective cleansers, their prolonged use can lead to scalp irritation, dryness, and environmental concerns. Consequently, there is a growing global demand for herbal cosmetics that offer safety, purity, and better biological compatibility. This study aimed to develop and evaluate an antimicrobial herbal shampoo powder using selected medicinal plants. The primary focus was to investigate how varying concentrations of antimicrobial herbs affect microbial growth inhibition while maintaining optimal physicochemical properties. Six formulations (F1–F6) were prepared using the trituration method. The herbal base consisted of Ocimum sanctum (Tulsi) and Azadirachta indica (Neem) as antimicrobial agents, with Sapindus trifoliatus (Soapnut) used for its natural saponins. Other supportive herbs included Amla, Hibiscus, Bhringraj, Henna, and Jujube. The formulations were evaluated for micromeritic properties, pH, foaming capacity, ash value, moisture content, and antimicrobial activity against Pityrosporum ovale and Escherichia coli. All formulations exhibited acceptable physical characteristics and a mildly acidic pH (4.9–6.0), which is suitable for scalp health. Micromeritic evaluation confirmed satisfactory flow properties for packaging. Antimicrobial activity was concentration-dependent; as the levels of Tulsi and Neem increased, the zone of inhibition also increased. Formulation F6, containing 32.5% Tulsi and 32.5% Neem, demonstrated the highest antimicrobial efficacy and superior foaming stability. Safety tests on volunteers and experimental animals showed the formulations were non-irritant to the skin with only mild, transient eye irritation. The study successfully developed a stable herbal shampoo powder that serves as a safe and effective alternative to synthetic products. Formulation F6 emerged as the most promising blend, combining maximum microbial inhibition with desirable cosmetic properties.

Keywords

Antimicrobial herbal shampoo, Ocimum sanctum, Micromeritic properties, pH, Foaming capacity, Ash value.

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    © The Author(s) 2025. This article is published by International Journal of Zoology and Applied Biosciences under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.