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International Journal of Zoology and Applied Biosciences Research Article
Optimization of cellulase production from Micrococcus sp using response surface methodology and evaluation of enzyme stability
P. Nirmala, S. Priya Grace and S. Gayathri
Year : 2026 | Volume: 11 | Issue: 3 | Pages: 224-233
Received on: 03/03/2026
Revised on: 20/03/2026
Accepted on: 22/04/2026
Published on: 01/05/2026
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P. Nirmala, S. Priya Grace and S. Gayathri( 2026).
Optimization of cellulase production from Micrococcus sp using response surface methodology and evaluation of enzyme stability
. International Journal of Zoology and Applied Biosciences, 11( 3), 224-233.
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Abstract
Cellulases are industrially important enzymes involved in the degradation of cellulose into fermentable sugars. The present study aimed to isolate and identify cellulase-producing bacteria from soil and optimize enzyme production using Response Surface Methodology (RSM). A total of ten bacterial isolates were obtained, among which five showed cellulolytic activity on CMC agar plates. Isolate S2 exhibited the highest enzyme activity (920.45 IU/mL) and was selected for further analysis. Morphological and biochemical characterization suggested that the isolate belongs to the genus Micrococcus. The enzyme demonstrated good stability under varying pH, metal ions, and organic solvents. Optimization using Central Composite Design significantly enhanced cellulase production, achieving a maximum activity of 1250.23 IU/mL at 72 h incubation, pH 7, and 1.5% inoculum. Statistical analysis confirmed model reliability. The findings highlight the potential of the isolate for industrial applications such as biofuel production and biomass conversion.
Keywords
Cellulase production; Micrococcus sp, Response Surface Methodology, Enzyme optimization,Soil bacteria.
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© The Author(s) 2025. This article is published by International Journal of Zoology and Applied Biosciences under the terms of the Creative Commons Attribution 4.0 International License (creativecommons.org), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
