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International Journal of Zoology and Applied Biosciences Research Article
Screening the silver nanoparticle synthesis potential of endophytic fungi with special reference to their antibacterial activity
Shyamji Shukla, Hemant Sahu, Harshita Shukla and Sardul Singh Sandhu
Year : 2024 | Volume: 9 | Issue: 6 | Pages: 19-28
Received on: 26/09/2024
Revised on: 10/10/2024
Accepted on: 22/10/2024
Published on: 19/11/2024
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Shyamji Shukla, Hemant Sahu, Harshita Shukla and Sardul Singh Sandhu( 2024).
Screening the silver nanoparticle synthesis potential of endophytic fungi with special reference to their antibacterial activity
. International Journal of Zoology and Applied Biosciences, 9( 6), 19-28.
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Abstract
Nanobiotechnology is an innovative field of science which involves biological synthesis, characterization and application of numerous nanoparticles. The present study was planned to explore potential of endophytic fungi for eco-friendly synthesis of silver nanoparticles with antibacterial activity. In this study 8 endophytic fungal isolates were obtained from different parts of medicinal plants. These isolates were identified belonging to different genera namely, Penicillium, Aspergillus, Alternaria etc. All endophytic fungi were subjected to extracellular biosynthesis of silver nanoparticles. Out of these Penicillium sp.,Aspergillus sp., Alternaria sp., isolated from healthy plant parts of Aegle marmelos, Dalbergia sissoo and Lantana camara respectively, gave positive results. The antibacterial activities of these nanoparticles were determined against some pathogenic bacteria by Agar Well Diffusion assay. During which it was found that the silver nanoparticles sample exhibiting highest antibacterial potential was characterized by UV-Visible Spectroscopy, Dynamic Light Scattering (DLS), Zeta Potential analysis and Transmission Electron Microscopy. UV-Visible spectroscopy analysis gave surface plasmone resonance peak alternatively 416nm, 444nm and 420nm.The average size range of silver nanoparticles as determined via, dynamic light scattering analysis is: 126nm, 88nm, 141nm. The Zeta potential of three samples as determined by zeta sizer showed a high negative surface charge on them which confers stability to them. The zeta potentials of these particles are: -19.1 mV, -22.0 mV, -16.2 mV. Silver nanoparticles produced by endophytic fungi displays considerable antibacterial agent and hence the study would prove to provide novel antibacterial agents.
Keywords
Antibacterial Activity, Biosynthesis, Characterization of Nanoparticle, Endophytic Fungi.
